Review



vectashield vibrance with dapi  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vectashield vibrance with dapi
    Vectashield Vibrance With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 804 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/H-1800/VECTASHIELD+Vibrance+Antifade+Mounting+Medium+with+DAPI/pm42844307-564-18-22
    Average 99 stars, based on 804 article reviews
    vectashield vibrance with dapi - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Blocking Assay:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Concentration Assay:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Plasmid Preparation:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Western Blot:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Sample Prep:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Electrophoresis:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Membrane:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Pore Size:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Purification:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Virus:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Expressing:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Mutagenesis:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Recombinant:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Electron Microscopy:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Control:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Modification:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Cell Isolation:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Transfection:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Reverse Transcription:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    SYBR Green Assay:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Microarray:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    RNA Sequencing Assay:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Knock-In:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Sequencing:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Software:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Flow Cytometry:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Microscopy:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.

    Real-time Polymerase Chain Reaction:

    Article Title: Additional Table 1
    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , N/A , Vector laboratories; H-1800 , IF; N/A.

    Article Title:
    Article Snippet: Vectashield Vibrance® Antifade Mounting Medium with DAPI , Vector Laboratories , H-1800.

    Article Title:
    Article Snippet: VECTASHIELD mounting medium , Vector laboratories , H1800.

    Article Title: KEY RESOURCES TABLE
    Article Snippet: Vectashield Vibrance anti-fade mounting media , Vector Labs , Cat# H-1800.

    Article Title:
    Article Snippet: Antifade Mounting Medium with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Antifade Mounting Media with DAPI , Vector , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance mounting media with DAPI , Vector Laboratories , Cat# H-1800.

    Article Title:
    Article Snippet: Vectashield Vibrance Antifade Mounting Medium with DAPI , Vector laboratories , Cat. No. H-1800.



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    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei <t>(DAPI;</t> blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .
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    Image Search Results


    Journal: iScience

    Article Title: Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells

    doi: 10.1016/j.isci.2026.117658

    Figure Lengend Snippet:

    Article Snippet: VECTASHIELD Antifade Mounting Media , Vector Laboratory , H-1800.

    Techniques: Cell-Signaling, Magnetic Beads, Virus, Recombinant, ROS Assay, Reverse Transcription, SYBR Green Assay, Staining, Plasmid Preparation, Flow Cytometry, Western Blot, Expressing, Control, Software

    Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei (DAPI; blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: Characterization of morphology, size, and shape distribution of differentiated 3D enteroids in suspension (A) Representative micrographs of AO enteroids: bright-field microscopy shows live enteroids in suspension (left), scanning electron microscopy (SEM) reveals a dense microvillar surface (middle), and transmission electron microscopy (TEM) shows the organized microvilli-rich brush border, tight junctions (TJ), and glycocalyx (right). High-magnification brightfield and SEM images (bottom row) correspond to the areas outlined by dotted squares in the upper row. (B) Representative micrographs of BO enteroids: live bright-field microscopy illustrates typical basal-out morphology (left), SEM shows the smooth basal surface (middle), and TEM reveals the microvilli-rich brush border on the lumen-facing apical surface, confirming basal-out polarity (right). High-magnification views (bottom row) correspond to the areas outlined in the upper row. (C) Circularity analysis of AO ( n = 118) and BO enteroids ( n = 94) in suspension from brightfield micrographs. (D) Total cross-sectional surface area at 6 days of suspension culture for AO ( n = 122) and BO ( n = 94) enteroids. (E) Concentrations of Matrigel proteins (% of protein over total acquired protein) present in AO and BO enteroids in suspension cultures after washing. Data from different donors ( n = 4, Hu22-2jej, Hu22-3jej, Hu22-5jej, and Hu18-9jej) are shown as means +SD. (F) Representative confocal images following staining of zonula occludens-1 (ZO-1, green), phalloidin (F-actin; magenta) and nuclei (DAPI; blue), highlighting the continuous monolayer barrier in AO and BO enteroids. Scale bars, 20 μm. (G) Quantification of apical-out and basal-out polarity in enteroid suspensions by confocal microscopy ( n = 100 per population). (A–D, F–G). Donor Hu18-9jej was used throughout. See also ; and .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Morphology, Suspension, Microscopy, Scanning Electron Microscopy, Transmission Assay, Electron Microscopy, Brightfield, Analysis, Confocal, Staining

    Fatty acid transport in 3D enteroids in suspension (A) Representative confocal images following staining with DAPI (nuclei; blue), SLC27A4 (solute carrier family 27 member 4, green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the fatty acid transport protein in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B and C) AO and BO enteroids were incubated with fluorescent fatty acid analog C1-BODIPY-C12 for 10 min and imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. (B) C1-BODIPY-C12 accumulated in fluorescent lipid droplets in AO enteroids. Scale bars, 10 μm. (C) BO enteroids showed intraluminal accumulation of C1-BODIPY-C12. Scale bars, 20 μm. (D) AO enteroids (donor Hu18-9jej) were co-incubated with fluorescent fatty acid analog C1-BODIPY-C12 and LipidTOX-DR for 10 minto visualize lipid droplets. AO enteroids were imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. Scale bars, 20 μm; inset scale bars, 10 μm. (A–D) Enteroids from donor Hu18-9jej were used. See also .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: Fatty acid transport in 3D enteroids in suspension (A) Representative confocal images following staining with DAPI (nuclei; blue), SLC27A4 (solute carrier family 27 member 4, green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the fatty acid transport protein in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B and C) AO and BO enteroids were incubated with fluorescent fatty acid analog C1-BODIPY-C12 for 10 min and imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. (B) C1-BODIPY-C12 accumulated in fluorescent lipid droplets in AO enteroids. Scale bars, 10 μm. (C) BO enteroids showed intraluminal accumulation of C1-BODIPY-C12. Scale bars, 20 μm. (D) AO enteroids (donor Hu18-9jej) were co-incubated with fluorescent fatty acid analog C1-BODIPY-C12 and LipidTOX-DR for 10 minto visualize lipid droplets. AO enteroids were imaged live using widefield fluorescence microscopy. The dashed square indicates the region shown at higher magnification. Scale bars, 20 μm; inset scale bars, 10 μm. (A–D) Enteroids from donor Hu18-9jej were used. See also .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Suspension, Confocal, Staining, Expressing, Incubation, Widefield, Fluorescence, Microscopy

    BCRP expression, localization and activity in AO enteroids in suspension (A) Schematic illustrating BCRP efflux under physiological function, inhibition or absence (e.g., knockout). (B) Representative confocal images following staining with DAPI (nuclei; blue), BCRP (green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the BCRP transporter in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (C) BCRP efflux in AO enteroids (donor Hu18-9jej) calculated by PhA accumulation; transporter substrate: PhA, transporter inhibitor: KO143. Results plotted as mean ± SD. Data are shown as triplicates in three independent experiments; data points of each independent experiment denoted as an individual shape.

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: BCRP expression, localization and activity in AO enteroids in suspension (A) Schematic illustrating BCRP efflux under physiological function, inhibition or absence (e.g., knockout). (B) Representative confocal images following staining with DAPI (nuclei; blue), BCRP (green) and phalloidin (F-actin; magenta), highlighting the expression pattern of the BCRP transporter in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (C) BCRP efflux in AO enteroids (donor Hu18-9jej) calculated by PhA accumulation; transporter substrate: PhA, transporter inhibitor: KO143. Results plotted as mean ± SD. Data are shown as triplicates in three independent experiments; data points of each independent experiment denoted as an individual shape.

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Expressing, Activity Assay, Suspension, Inhibition, Knock-Out, Confocal, Staining

    CYP3A4 metabolic activity in AO enteroids in suspension and PBPK simulation (A) Representative confocal images following staining with DAPI (nuclei; blue), CYP3A4 (green), and phalloidin (F-actin; magenta), highlighting the expression pattern of the CYP3A4 metabolic enzyme in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B) CYP3A4 metabolic activity in AO enteroids (donor Hu18-9jej) over a time period of 120 min. The concentration of the enzyme substrate terfenadine was monitored over time, as well as the formation of its metabolite fexofenadine. Data are shown as mean ± SD. (C) Intrinsic clearance of terfenadine in AO enteroids (donor: Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej). Data are shown as median ± IQR, ∗∗∗ p < 0.001 (Mann-Whitney U test). (D) Corrected intrinsic clearance of terfenadine considering the differences in CYP3A4 protein expression in AO enteroids (donor Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej. Data are shown as median ± IQR. Mann-Whitney U test showed no statistically significant difference between groups. (E) Simulated mean pharmacokinetics profile of a 60 mg dose of terfenadine in 140 virtual males (20–50 years, black line) with its 5th and 95th percentile profile shown as dashed lines. The green line represents the PBPK model mean prediction for AO enteroids (log concentration). The orange dots depict mean observed data from Okerholm et al. See also ; .

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet: CYP3A4 metabolic activity in AO enteroids in suspension and PBPK simulation (A) Representative confocal images following staining with DAPI (nuclei; blue), CYP3A4 (green), and phalloidin (F-actin; magenta), highlighting the expression pattern of the CYP3A4 metabolic enzyme in AO enteroids (donor Hu18-9jej). Scale bars, 20 μm. (B) CYP3A4 metabolic activity in AO enteroids (donor Hu18-9jej) over a time period of 120 min. The concentration of the enzyme substrate terfenadine was monitored over time, as well as the formation of its metabolite fexofenadine. Data are shown as mean ± SD. (C) Intrinsic clearance of terfenadine in AO enteroids (donor: Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej). Data are shown as median ± IQR, ∗∗∗ p < 0.001 (Mann-Whitney U test). (D) Corrected intrinsic clearance of terfenadine considering the differences in CYP3A4 protein expression in AO enteroids (donor Hu18-9jej) and primary enterocytes (donors: Hu22-32jej, Hu23-1jej and Hu23-5jej. Data are shown as median ± IQR. Mann-Whitney U test showed no statistically significant difference between groups. (E) Simulated mean pharmacokinetics profile of a 60 mg dose of terfenadine in 140 virtual males (20–50 years, black line) with its 5th and 95th percentile profile shown as dashed lines. The green line represents the PBPK model mean prediction for AO enteroids (log concentration). The orange dots depict mean observed data from Okerholm et al. See also ; .

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Activity Assay, Suspension, Confocal, Staining, Expressing, Concentration Assay, MANN-WHITNEY, Drug discovery

    Journal: iScience

    Article Title: Proteomic and functional profiling of suspended jejunal enteroids reveals pharmacokinetic fidelity to native intestine

    doi: 10.1016/j.isci.2026.117654

    Figure Lengend Snippet:

    Article Snippet: VECTASHIELD Vibrance Antifade Mounting Medium with DAPI , Vector Laboratories , Cat #: H-1800–10.

    Techniques: Tissue, Recombinant, Membrane, Gentle, Cell Recovery, Staining, Plasmid Preparation, Saline, Viability Assay, Lysis, Mass Spectrometry, Software

    Photoreceptor labeling in (A–C) Normal ERG phenotype, (D–F) cone dysfunction phenotype, and (G–I) severe retinal dysfunction phenotype animals. (A, D, G) Representative fluorescence microscopy images of retinal sections labeled for anti-Cone Arrestin ( red ). (B, E, H) Representative fluorescence microscopy images of retinal sections labeled anti-Rhodopsin ( green ). (C, F, I) Labeling of cell nuclei with DAPI ( blue ) is shown as merged images with anti-Cone Arrestin and anti-Rhodopsin. (J) Percentage of retinal area positive for cone arrestin. (K) Percentage of retinal area positive for rhodopsin. In cone dysfunction phenotype animals the cone band shows irregularities and retraction of its outer segments, and the rod external segments band is similar than the normal ERG phenotype. In severe retinal dysfunction phenotype animals the cone band has almost disappeared, and the rod band is absent. ** P < 0.01; **** P < 0.0001. Two-way ANOVA with Tukey multiple comparisons.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Spontaneous Achromatopsia-Like Phenotype in Albino Swiss CD-1 Mice: Retinocortical Dysfunction, Neurovascular Remodeling, and Glial Activation

    doi: 10.1167/iovs.67.12.20

    Figure Lengend Snippet: Photoreceptor labeling in (A–C) Normal ERG phenotype, (D–F) cone dysfunction phenotype, and (G–I) severe retinal dysfunction phenotype animals. (A, D, G) Representative fluorescence microscopy images of retinal sections labeled for anti-Cone Arrestin ( red ). (B, E, H) Representative fluorescence microscopy images of retinal sections labeled anti-Rhodopsin ( green ). (C, F, I) Labeling of cell nuclei with DAPI ( blue ) is shown as merged images with anti-Cone Arrestin and anti-Rhodopsin. (J) Percentage of retinal area positive for cone arrestin. (K) Percentage of retinal area positive for rhodopsin. In cone dysfunction phenotype animals the cone band shows irregularities and retraction of its outer segments, and the rod external segments band is similar than the normal ERG phenotype. In severe retinal dysfunction phenotype animals the cone band has almost disappeared, and the rod band is absent. ** P < 0.01; **** P < 0.0001. Two-way ANOVA with Tukey multiple comparisons.

    Article Snippet: After the final washes (three times in PBS), sections were mounted using Vectashield Vibrance Antifade medium with DAPI (Ref. H-1800; Vector Laboratories), and retinal whole-mounts were mounted using Vectashield Vibrance Antifade medium without DAPI (Ref. H-1700; Vector Laboratories).

    Techniques: Labeling, Fluorescence, Microscopy

    Bipolar and RGC labeling in (A–C) Normal ERG phenotype, (D–F) cone dysfunction phenotype, and (G–I) severe retinal dysfunction phenotype animals. (A, D, G) Representative fluorescence microscopy images of retinal sections labeled for anti-PKCα ( red ). (B, E, H) Representative fluorescence microscopy images of retinal sections labeled with anti-Brn3a ( green ). (C, F, I) Labeling of cell nuclei with DAPI ( blue ) is shown as merged images with anti-PKCα and anti-Brn3a. (J) Vertical extent of PKCα+ bipolar cells (µm). (K) Regional (central, intermediate, peripheral) analysis of the vertical extent of PKCα+ bipolar cells (µm). (L) Brn3a+ retinal ganglion cell counts. In cone dysfunction phenotype and severe retinal dysfunction phenotype animals, the rod bipolar cell band appears reduced in length, although its overall structure remains preserved. Brn3a+ RGC density is reduced in both groups with visual impairment, consistent with quantitative Brn3a+ cell counts. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Two-way ANOVA with Tukey multiple comparisons.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Spontaneous Achromatopsia-Like Phenotype in Albino Swiss CD-1 Mice: Retinocortical Dysfunction, Neurovascular Remodeling, and Glial Activation

    doi: 10.1167/iovs.67.12.20

    Figure Lengend Snippet: Bipolar and RGC labeling in (A–C) Normal ERG phenotype, (D–F) cone dysfunction phenotype, and (G–I) severe retinal dysfunction phenotype animals. (A, D, G) Representative fluorescence microscopy images of retinal sections labeled for anti-PKCα ( red ). (B, E, H) Representative fluorescence microscopy images of retinal sections labeled with anti-Brn3a ( green ). (C, F, I) Labeling of cell nuclei with DAPI ( blue ) is shown as merged images with anti-PKCα and anti-Brn3a. (J) Vertical extent of PKCα+ bipolar cells (µm). (K) Regional (central, intermediate, peripheral) analysis of the vertical extent of PKCα+ bipolar cells (µm). (L) Brn3a+ retinal ganglion cell counts. In cone dysfunction phenotype and severe retinal dysfunction phenotype animals, the rod bipolar cell band appears reduced in length, although its overall structure remains preserved. Brn3a+ RGC density is reduced in both groups with visual impairment, consistent with quantitative Brn3a+ cell counts. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Two-way ANOVA with Tukey multiple comparisons.

    Article Snippet: After the final washes (three times in PBS), sections were mounted using Vectashield Vibrance Antifade medium with DAPI (Ref. H-1800; Vector Laboratories), and retinal whole-mounts were mounted using Vectashield Vibrance Antifade medium without DAPI (Ref. H-1700; Vector Laboratories).

    Techniques: Labeling, Fluorescence, Microscopy, Analysis

    Characteristics of microglial cells and astrocytes/ Müller glia in retinal sections in the different study groups: (A–C) Normal ERG phenotype, (D–F) cone dysfunction phenotype, and (G–I) severe retinal dysfunction phenotype animals. (A, D, G) Representative fluorescence microscopy images of retinal sections labeled for anti-Iba-1 (specific of microglia) ( red ) and (B, E, H) anti-GFAP (specific of astrocytes and reactive Müller glia) ( green ) in normal ERG phenotype, cone dysfunction phenotype and severe retinal dysfunction phenotype animals. (C, F, I) Labeling of cell nuclei with DAPI ( blue ) is shown as merged images with anti-Iba and anti-GFAP. (J) Percentage of retinal area positive for Iba1. (K) Percentage of retinal area positive for GFAP. (D, F) In cone dysfunction phenotype animals, Iba1 immunoreactivity increased in these layers, indicating higher cell density and thicker cells bodies and processes, consistent with microglial activation. (G, I) In severe retinal dysfunction phenotype animals, Iba1+ microglia are increased and show a hypertrophic, retracted morphology, and the retina is thinner because of loss of the outer layers. (E, F) In cone dysfunction phenotype animals, a thicker GFAP+ band is observed compared to the normal ERG phenotype group. (H, I) In severe retinal dysfunction phenotype animals, in addition to a prominent GFAP+ band, GFAP+ vertical Müller glia columns ( arrowhead ) are also observed. * P < 0.05; ** P < 0.01; **** P < 0.0001. Two-way ANOVA with Tukey multiple comparisons. Scale bar : 50 µm.

    Journal: Investigative Ophthalmology & Visual Science

    Article Title: Spontaneous Achromatopsia-Like Phenotype in Albino Swiss CD-1 Mice: Retinocortical Dysfunction, Neurovascular Remodeling, and Glial Activation

    doi: 10.1167/iovs.67.12.20

    Figure Lengend Snippet: Characteristics of microglial cells and astrocytes/ Müller glia in retinal sections in the different study groups: (A–C) Normal ERG phenotype, (D–F) cone dysfunction phenotype, and (G–I) severe retinal dysfunction phenotype animals. (A, D, G) Representative fluorescence microscopy images of retinal sections labeled for anti-Iba-1 (specific of microglia) ( red ) and (B, E, H) anti-GFAP (specific of astrocytes and reactive Müller glia) ( green ) in normal ERG phenotype, cone dysfunction phenotype and severe retinal dysfunction phenotype animals. (C, F, I) Labeling of cell nuclei with DAPI ( blue ) is shown as merged images with anti-Iba and anti-GFAP. (J) Percentage of retinal area positive for Iba1. (K) Percentage of retinal area positive for GFAP. (D, F) In cone dysfunction phenotype animals, Iba1 immunoreactivity increased in these layers, indicating higher cell density and thicker cells bodies and processes, consistent with microglial activation. (G, I) In severe retinal dysfunction phenotype animals, Iba1+ microglia are increased and show a hypertrophic, retracted morphology, and the retina is thinner because of loss of the outer layers. (E, F) In cone dysfunction phenotype animals, a thicker GFAP+ band is observed compared to the normal ERG phenotype group. (H, I) In severe retinal dysfunction phenotype animals, in addition to a prominent GFAP+ band, GFAP+ vertical Müller glia columns ( arrowhead ) are also observed. * P < 0.05; ** P < 0.01; **** P < 0.0001. Two-way ANOVA with Tukey multiple comparisons. Scale bar : 50 µm.

    Article Snippet: After the final washes (three times in PBS), sections were mounted using Vectashield Vibrance Antifade medium with DAPI (Ref. H-1800; Vector Laboratories), and retinal whole-mounts were mounted using Vectashield Vibrance Antifade medium without DAPI (Ref. H-1700; Vector Laboratories).

    Techniques: Fluorescence, Microscopy, Labeling, Activation Assay, Morphology